赫利俄斯/UltraGRO™ GMP级/HPCPLCGL50、HPCPLCGL10、HPCPLCGL05
品牌：
Helios
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Overview
- Application
- Safety Information
Description:
UltraGRO™ cell culture supplement is a non-xenogeneic, animal serum-free, and heparin-requiring media supplement for replacing FBS (fetal bovine serum) to support cell expansion from research through clinical trials to commercial use. UltraGRO™ contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs).
Specification:
1. Appearance: Yellow to Buff liquid2. Total Protein: 4.0 - 8.0 g/dL3. pH: 6.5 - 8.54. Osmolality: 275 - 355 mOsm/Kg5. Endotoxin: ≤ 10 EU/mL6. Mycoplasma: Not Detected7. Sterility: No Growth8. Performance Test: Test and record
Storage and Shipping Information:
UltraGRO™ is most stable when stored frozen until needed. The recommended storage temperature is -20°C or -80°C. Thaw frozen UltraGRO™ product in 37°C water bath before use. Once UltraGRO™ is thawed, it is recommended to fully use for completed medium preparation (e.g. 5%) the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C or -80°C.
Packaging Information:
500mL, 100mL, 50mL
Media Form:
Liquid
Application:
For human ex-vivo tissue and cell culture processing applications.
Instructions for Use:
1. UltraGRO™ shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.2. We recommend seeding MSCs at approximately 3x103~6x103 per cm2.3. UltraGRO™ requires heparin at a final concentration of 2 IU/mL to be added in the culture media when supplemented with 5% UltraGRO™. Failure to add heparin will result in coagulation during cell culture in typical media.
Cell Lines:
Bone marrow mesenchymal stem cells, Adipose tissue derived mesenchymal stem cells, Umbilical cord derived mesenchymal stem cells, Other mesenchymal stem cells.
Important Information:
1. Clotting or insoluble particles may form in thawed UltraGRO™ cell culture supplement. Published research has show that particles will not alter the performance of the product.2. Clotting or insoluble particles may form in thawed UltraGRO™, it is recommended to centrifuge at 3,400 xg for 3~5 minutes or to filter the liquid concentrate with a sterile 40 μm Cell Strainer to remove insoluble particles.3. Filtering the completed medium (e.g. 5%), after UltraGRO™ is diluted in the basal medium, will not affect UltraGRO™ supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™ concentrate, as this may reduce 5% UltraGRO™ cell culture performance.4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™ performance.
Safety Packaging information:
1. Follow the handling instructions outlined in the Material Safety Date Sheets (MSDSs). Wear appropriate protective eyewear, clothing, and gloves.2. UltraGRO™ is a cell culture supplement derived from human platelets collected from healthy donors at FDA or HC licensed blood centers. Each donor has been tested using FDA/HC-licensed tests and found nonreactive for HBsAg, Hepatitis B core antibody (anti-HBc), HIV antibody (anti-HIV-1/2), Hepatitis C antibody (anti-HCV), HTLV-1/2 antibody (anti-HTLV-1/2), Trypanosoma cruzi antibody (anti-T. cruzi), HIV-1, HCV, HBV, WNV nucleic acid testing and Syphilis microhemagglutination test. Each donation is tested for ZIKV under either and FDA licensed test or an investigational ID-NAT test and sound non-reactive.3. This product is manufactured, tested and released in compliance with the relevant GMP guidelines. This product is for in vitro use only.
品牌介绍
AventaCell 是致力于开发新的用于细胞培养和组织再生领域的人类衍生产品。Helios 是 AventaCell 的旗下品牌，其产品用于细胞培养和组织再生。Helios 生物科学产品被设计用于支持广泛的细胞的扩增和生产，包括间充质干细胞和多个免疫细胞系。Helios 生物科学产品优势：无动物成分添加/血清替代品用量仅5%，即可获得比20% FBS更快生长速度在原代隔离与扩增培养中有更好的表现缩短细胞翻倍时间(20-30小时)生产百万细胞的费用都可以有效降低无需细胞附着因子批次间一致性高无需肝素GMP级的产品生产及检测环境HELIOS产品生产流程检测步骤均在B级洁净区进行。产品优点:仅用5％UltraGRO™/ UltraGRO™-Advanced / UltraGRO™-PURE替代高达20％的FBS在初级和扩展文化中表现更好缩短人口倍增时间（20~30小时）产生的每百万细胞成本更低无需粘附因子可重复的细胞培养结果从临床前到临床试验，使用相同的补充剂UltraGRO™-Advanced不需要肝素药物主文件可用于UltraGRO™-Advanced / UltraGRO™-PUREUltraGRO™-PURE中100％无异物
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